Plant Transcription Factor Database
Previous version: v3.0
Nicotiana benthamiana
FAR1 Family
Species TF ID Description
Niben101Scf00163g06003.1FAR1 family protein
Niben101Scf00163g06010.1FAR1 family protein
Niben101Scf00217g02002.1FAR1 family protein
Niben101Scf00217g02009.1FAR1 family protein
Niben101Scf00304g06001.1FAR1 family protein
Niben101Scf01013g00001.1FAR1 family protein
Niben101Scf01124g16011.1FAR1 family protein
Niben101Scf01142g00014.1FAR1 family protein
Niben101Scf01258g01001.1FAR1 family protein
Niben101Scf01462g01002.1FAR1 family protein
Niben101Scf01623g04023.1FAR1 family protein
Niben101Scf01623g10008.1FAR1 family protein
Niben101Scf01670g05002.1FAR1 family protein
Niben101Scf01683g17031.1FAR1 family protein
Niben101Scf01734g01022.1FAR1 family protein
Niben101Scf01734g01038.1FAR1 family protein
Niben101Scf01734g01062.1FAR1 family protein
Niben101Scf01739g03001.1FAR1 family protein
Niben101Scf02474g03002.1FAR1 family protein
Niben101Scf02486g03001.1FAR1 family protein
Niben101Scf02807g11010.1FAR1 family protein
Niben101Scf02807g11013.1FAR1 family protein
Niben101Scf03004g02017.1FAR1 family protein
Niben101Scf03332g02010.1FAR1 family protein
Niben101Scf03561g01010.1FAR1 family protein
Niben101Scf03705g02012.1FAR1 family protein
Niben101Scf04464g02002.1FAR1 family protein
Niben101Scf04953g06005.1FAR1 family protein
Niben101Scf05178g00002.1FAR1 family protein
Niben101Scf06392g02008.1FAR1 family protein
Niben101Scf08448g00001.1FAR1 family protein
Niben101Scf08954g02005.1FAR1 family protein
Niben101Scf09100g04008.1FAR1 family protein
Niben101Scf09640g00011.1FAR1 family protein
Niben101Scf13694g00021.1FAR1 family protein
Niben101Scf15657g00001.1FAR1 family protein
Niben101Scf17255g00011.1FAR1 family protein
Niben101Scf17594g00002.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885